| 產品名稱 |
pro-caspase-3 |
| 商品貨號 |
B161776 |
| Designations |
pro-caspase-3 |
| GenBank Number |
U26943
|
| Species |
Homo sapiens, human |
| Depositors |
G Salvesen |
| Applications |
in another host, produces protein apopain in another host, produces protein caspase 3, apoptosis-related cysteine protease Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease), cysteine protease CPP32 |
| Vector |
Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pET-23b
Intact vector size: 3.665
Type of vector: plasmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: BamHI EcoRI SacI HincII SalI HindIII EagI NotI XhoI
Polylinker sites: BamHI EcoRI SacI HincII SalI HindIII EagI NotI XhoI
Other unique sites: NheI NdeI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
transcription terminator: T7, <-, 26-72
epitope tag: hexahistidine sequence (6-His), <-, 140-157
MCS: BamHI...XhoI, <-, 158-203
epitope tag: T7 tag sequence, <-, 207-239
promoter for in vitro transcription: T7, <-, 303-319
replicon: pMB1, 1450-1450
marker(s): ampR, <-, 2211-3068
replicon: f1, <-, 3200-3655
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CASP3
Other gene symbols: CPP32; CPP32B; Yama; apopain
Gene name: caspase 3, apoptosis-related cysteine protease
Contains complete coding sequence?: Y
Chromosome: 4; Localization: 4 q34
Tissue: umbilical vein endothelial cells
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 0.9
Cross references: DNA Seq. Acc.: U26943
Nucleotides ~1-900 of the insert correspond to
nucleotides ~1-834 of U26943. Insert lengths(kb): 0.8999999761581421 Tissue: umbilical vein endothelial cells Gene product: caspase 3, apoptosis-related cysteine protease( Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease), cysteine protease CPP32) [Yama] Target Gene: apopain, caspase 3, apoptosis-related cysteine protease |
| Insert Size (kb) |
0.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): NdeI--4.5; XbaI/XhoI--4.0, 0.95; HindIII--4.5. Plasmid encodes the complete coding sequence, tagged at the C-terminus with hexahistidine. |
| References |
Orth K, et al. Molecular ordering of apoptotic mammalian CED-3/ICE-like proteases. J. Biol. Chem. 271: 20977-20980, 1996. PubMed: 8702858
Quan LT, et al. Proteolytic activation of the cell death protease Yama/CPP32 by granzyme B. Proc. Natl. Acad. Sci. USA 93: 1972-1976, 1996. PubMed: 8700869
Stennicke HR, Salvesen GS. Biochemical characteristics of caspases-3, -6, -7, and -8. J. Biol. Chem. 272: 25719-25723, 1997. PubMed: 9325297
|
