| 產品名稱 |
mp35 |
| 商品貨號 |
B161990 |
| Designations |
mp35 |
| GenBank Number |
M86672
|
| Species |
Mus musculus, mouse |
| Depositors |
U Gubler |
| Vector |
Construct size (kb): 3.664000034332275 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il12a
Genomic copy number: unique
Gene name: interleukin 12A (natural killer cell stimulatory factor 1,
cytotoxic lymphocyte maturation factor 1, p35
Contains complete coding sequence?: Y
Chromosome: 3
Strain: C57BL/6
Tissue: spleen
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: NotI
Insert size (kb): 0.7
Cross references: DNA Seq. Acc.: M86672
Nucleotides 54 - 687 of the insert correspond to
nucleotides 131 - 774 of M86672. Insert lengths(kb): 0.699999988079071 Tissue: spleen Gene product: interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35 [Il12a] |
| Insert Size (kb) |
0.700 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/NotI--2.9, 0.78; XbaI--2.9, 0.50, 0.28; HindIII--3.7. The insert contains the following restriction sites (approximate kb from the 5' end): XbaI--0.49; StyI--0.40; SacI--0.68. The sequence surrounding the initiation codon was modified to 5'-ACCAUGG-3' (Kozak consensus) to optimize translation. Thus the sequence between the vector insertion site and the initiating ATG is not derived from the mRNA. The p35 cDNA was cloned into pBluescript SK+ via HindIII to BamHI (Aug to stop), without recreating the BamHI site. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Schoenhaut DS, et al. Cloning and expression of murine IL-12. J. Immunol. 148: 3433-3440, 1992. PubMed: 1350290
|
