Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC.
Product Format
frozen
Storage Conditions
?Store at -70°C or colder
Comments
This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC.
Recommended Host
Production Host:ST cells (ATCC CRL-1746)
Recommended Host: ?ST cells (ATCC? CRL-1746?)
Growth Conditions
Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and rinse cells twice with VGM without TPCK-treated trypsin. Leave the second wash over the cells for 3 hours at 37.0°C in a humidified 5% CO2 atmosphere. Remove the second wash from the cells and then inoculate with a small volume of virus diluted with VGM supplemented with TPCK- treated trypsin (1 μg/ mL) to provide a MOI of about 0.1. Adsorb for 1 hour at 37.0°C in a humidified 5% CO2 atmosphere. End adsorption by adding VGM supplemented with 1 μg/ mL of TPCK- treated trypsin.
Incubate infected culture for 3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 70-80% of the culture.
Name of Depositor
LJ Saif
Source
Small intestine of neonatal pig with porcine transmissible gastroenteritis.
References
Bohl EH, et al. Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect. Immun. 6: 289-301, 1972. PubMed: 4629259
Bohl EH, Kumagai T. The use of cell cultures for the study of TGE virus of swine. Proc. U.S. Livestock Sanit. Assoc. 69: 343-350, 1965.
