| 產品名稱 |
6F4C5 |
| 商品貨號 |
B162006 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rat Golgi complex (GCI) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with a galactosyl transferase rich microsomal fraction of rat myeloma cells. Spleen cells were fused with Sp2/0 myeloma cells. The antibody is directed against an antigen localized on the cytoplasmic face of the Golgi membrane. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
AM Tartakoff, Y Chicheportiche |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Chicheportiche Y, et al. Characterization of cytoplasmically oriented Golgi proteins with a monoclonal antibody. J. Cell Biol. 99: 2200-2210, 1984. PubMed: 6438114
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