| Vector |
Construct size (kb): 48.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D14S308
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.3
Genome: Homo sapiens
Gene symbol: IGHV@
Genomic copy number: unique
Gene name: immunoglobulin heavy polypeptide, variable region (cluster)
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.33
Tissue: leukocyte
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 40.0
Cross references: DNA Seq. Acc.: Z30083 Insert lengths(kb): 40.0 Tissue: leukocyte Gene product: immunoglobulin heavy-chain variable region [IGHV@] |
| Comments |
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Contains the 8.5 kb EcoRI fragment encoding V(H) 5-1. Subcloned from yRM2006 (ATCC 99425). One of a set of 2 cosmid contigs ordered, from the telomeric side, yIgH6c10, 2, 4, 9, 5, 14, 3, 19, 1, and 18. The gap is between 9 and 5. These cosmids have been assigned ATCC numbers 99460, 99452, 99456, 99458, 99472, 99462, 99454, 99466, 99450, and 99464, respectively. |
