| Insert |
DNA: cDNA Insert lengths(kb): 8.574999809265137 Gene product: von Willebrand factor(coagulation factor VIII VWF (von Willebrand factor)) [VWF] Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2 Target Gene: von Willebrand factor |
| Comments |
The insert contains the following restriction sites (nt position according to the published sequence): SacII--1614, 1991, 7618; BglII--2240, 6935; HindIII--2334; BamHI--2816. Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 5.5; BamHI--6.7, 3.7, 3.25, 0.5; BglII--7.0, 4.9, 2.35; HindIII--10.8, 2.5, 0.95. Contains the complete coding sequence (nt 100 to nt 8541), with the mature peptide encoded from nt 2389 to nt 8538. May be transfected into COS cells for transient expression of an immunoreactive product detected in conditioned medium. The vector contains the SV40 ori, the adenovirus major late promoter and tripartite leader, the SV40 polyadenylation signal, the adenovirus VA I and VA II genes, and pBR322 sequences. |
| References |
Bonthron DT, et al. Structure of pre-pro-von Willebrand factor and its expression in heterologous cells. Nature 324: 270-273, 1986. PubMed: 3491324
Wise RJ, et al. The propeptide of von Willebrand factor independently mediates the assembly of von Willebrand multimers. Cell 52: 229-236, 1988. PubMed: 3124962
Bonthron D, et al. Nucleotide sequence of pre-pro-von Willebrand factor cDNA. Nucleic Acids Res. 14: 7125-7127, 1986. PubMed: 3489923
D'Andrea A, et al. Recombinant DNA encoding an erythropoietin receptor. US Patent 5,278,065 dated Jan 11 1994
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