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CRE BAG 2

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編號:B162306

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產品名稱	CRE BAG 2
商品貨號	B162306
Organism	Mus musculus, mouse
Tissue	embryo
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Leukemia
Strain	NIH/Swiss
Applications	produces protein beta-galactosidase beta-D-galactosidase
Derivation	The CRE BAG 2 cell line has never produced a replication competent helper virus. The cells will package vectors derived from murine leukemia viruses.
Genes Expressed	a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Cellular Products	a beta galactosidase transducing vector (BAG)
Comments	The BAG construct has been used to transfect psiCRE cells in ATCC CRL-1858 and to transfect psi2 cells in ATCC CRL-9560. The CRE BAG 2 cell line has never produced a replication competent helper virus. This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity. The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus. The cells will package vectors derived from murine leukemia viruses. The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions	Temperature: 37.0°C
Name of Depositor	CL Cepko
References	Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292 Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789 Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107 Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989. Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284

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