Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 1.35. The actin promoter has been shown to be expressed efficiently in undifferentiated cells in which transcription from other promoters (SV40 early, Mo MuLV LTR, MMTV LTR) is severely repressed. A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the rat beta actin promoter. Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and the rat beta-actin promoter (-340 to +10). |
