| 產品名稱 |
SIRC [Statens Seruminstitut Rabbit Cornea] |
| 商品貨號 |
B162402 |
| Organism |
Oryctolagus cuniculus, rabbit |
| Tissue |
cornea |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
This cell line is suitable for primary isolation of rubella virus. The early appearance of distinct cytopathic changes makes this cell line highly suitable for both the propagation and quantitation of rubella virus. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 66; range = 51 to 90
The modal number (66) does not permit sexing of donor cytologically. Most cells contain one long, unpaired, submetacentric chromosome and 2 to 3 small telocentric chromosomes with satellites. Polyploidy was observed in 5/200 metaphase cells. |
| Derivation |
The SIRC cell line was derived by M. Volkert of the Staatens Seruminstitut, Copenhagen, Denmark, from the cornea of a normal rabbit in 1957. |
| Virus Susceptibility |
Rubella virus
,
Rubella virus
|
| Comments |
In 1965, J. Leerhoy found this line to be susceptible to rubella virus. C.A. Phillips, et al. confirmed the above findings and demonstrated the suitability of the cell line for primary isolation of rubella virus.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
J Leerhoy |
| Deposited As |
Oryctolagus cuniculus |
| Passage History |
Little is recorded relating to the history of this cell line for approximately the first 400 passages. |
| Year of Origin |
1957 |
| References |
Leerhoy J. Cytopathic effect of rubella virus in a rabbit-cornea cell line. Science 149: 633-634, 1965. PubMed: 14331182
Phillips CA, et al. Isolation, propagation and neutralization of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 122: 783-786, 1966. PubMed: 5918951
Rhim JS, et al. Plaque assays of rubella virus in cultures of rabbit cornea (SIRC) cells. Proc. Soc. Exp. Biol. Med. 125: 1271-1274, 1967. PubMed: 6042441
Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741
|
