| Vector |
Construct size (kb): 9.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: p3CLHygro
Intact vector size: 8.300
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: HindIII SalI
Polylinker sites: HindIII SalI
Construction: p3CL, pHEBo (hygR)
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, hygR
promoter: CMV
replicon: pMB1, SV40
terminator: bGH polyadenylation
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HTR1DA*
Gene name: 5-hydroxytryptamine (serotonin) receptor 1D alpha
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert end: SalI
Insert end: SalI
Insert size (kb): 1.1
Cross references: Insert lengths(kb): 1.100000023841858 Gene product: 5-hydroxytryptamine (serotonin) receptor 1D alpha [HTR1DA*] Target Gene: 5-hydroxytryptamine (serotonin) receptor 1D alpha |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--8.6, 0.92; BglII--7.8, 1.2, 0.48; HindIII--8.3, 0.92, 0.29; SalI--8.3, 1.2; XhoI--5.2, 4.3; EcoRI--6.0, 2.4, 1.0. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.09, 1.00; BglII--0.11, 0.58; HindIII--0.84, 1.10; XhoI--0.88. Contains the complete coding sequence with only a few extra nucleotides. The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - hygR - tk polyadenylation - TaqI - SV40 polyadenylation - EcoRI - CMV promoter >- HindIII - SalI - insert >- SalI - bGH polyadenylation - PvuI. |
