| Vector |
Construct size (kb): 6.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCR3
Intact vector size: 5.100
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: HindIII KpnI SacI BamHI SpeI XmaIII BstXI EcoRI EcoRI PstI
EcoRV BstXI NotI XhoI XbaI ApaI
Construction: pcDNA3
Features (with orientation and position when available):
promoter: T7, ->
MCS: HindIII/.../TA cloning site/.../ApaI, ->
promoter: SP6, <-
other: bovine growth hormone polyA signal, ->
replicon: pMB1
marker(s): kanR, <-
promoter: SV40, <-
replicon: SV40, <-
marker(s): ampR, <-
replicon: f1, ->
promoter: cytomegalovirus, ->
Cross references: |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; EcoRI--5.6, 1.5; XhoI--5.6, 0.82; NotI--7.0; SacI--5.2, 1.1, 0.52, 0.084. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.46; XhoI--0.65. |
