The insert contains the following sites separated by (bp)(approx): EcoRI- 270- SmaI- 160- PstI- 240- PstI- 210- PstI- 100- PstI- 530- KpnI- 310- PstI- 1080- HindIII- 110- BamHI- 205- PstI- 475- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 2.7; SacI--6.5; AvaI--3.4, 2.0, 0.2; XbaI--5.9, 0.54, 0.2, 0.1. The insert includes 146 nt of 5'-noncoding sequence, an open reading frame of 1725 nt and 1779 nt of 3'-noncoding sequence including 40 nt of poly(A) tail. There is 64% homology between this probe and bovine thrombomodulin. A single band of 3.7 kb is detected in human placenta and endothelial cell poly(A)+ RNA. |
