| Insert |
DNA: genomic Insert lengths(kb): 3.0 Gene product: DNA Segment, single copy [D11S144] Alleles: A1, A1, A1, A2, A2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7, 1.2, 0.94, 0.74; PstI--3.6, 1.7, 0.6; EcoRI/HindIII--3.0, 1.7, 0.82, 0.5; EcoRI--4.25, 0.87, 0.43. This probe was also known as D11S285. Codominant segregation demonstrated in 46 3-generation families. This probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal hybridization and wash conditions. Enzyme(s) not detecting polymorphism: BglII, PvuII, TaqI. |
| References |
Smith M, et al. Mapping of a gene determining tuberous sclerosis to human chromosome 11q14-11q23. Genomics 6: 105-114, 1990. PubMed: 2303253
Charmley P, et al. A primary linkage map of the human chromosome 11q22-23 region. Genomics 6: 316-323, 1990. PubMed: 2307473
Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence MCT128.1 on chromosome 11 [D11S285]. Nucleic Acids Res. 16: 378, 1988. PubMed: 2893342
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