| Vector |
Construct size (kb): 12.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-2
Intact vector size: 2.870
Type of vector: plasmid
Vector end: SalI
Vector end: XbaI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->, 10-60
promoter for in vitro transcription: T7, <-, 65-87
replicon: pMB1
marker(s): ampR, <-, 1098-1958
promoter for in vitro transcription: SP6, ->, 2852-2869
Cross references: DNA Seq. Acc.: X65301 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGF2R
Genomic copy number: unique
Gene name: insulin-like growth factor 2 receptor
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 q25.3
Tissue: placenta
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: XbaI
Insert size (kb): 9.1
Cross references: DNA Seq. Acc.: J03528 Insert lengths(kb): 9.100000381469727 Tissue: placenta Gene product: insulin-like growth factor 2 receptor( insulin-like growth factor II receptor, insulin like growth factor II (IGF II) receptor, mannose-6-phosphate receptor, cation-independent) [IGF2R] Alleles: A1, A1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0, 1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7; SalI/XhoI--9.0, 2.7. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34, 6.91. At the 3' end of the insert, the construct includes sequence from the vector pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation signal (to the SalI site). |
| References |
Oshima A, et al. The human cation-independent mannose 6-phosphate receptor. Cloning and sequence of the full-length cDNA and expression of functional receptor in COS cells. J. Biol. Chem. 263: 2553-2562, 1988. PubMed: 2963003
William S Sly, personal communication
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