Restriction digests of the clone give the following sizes (kb): EcoRI--10.0; HindIII--10.0; EcoRI/HindIII--7.2, 2.8; XbaI--uncut. This construct contains nt 3133-5840 (gene 1) of bacteriophage T7. M13mGP1-2 (ATCC 40303) can coexist in the same cell as the two plasmids of ATCC 67287, pGP5-5 and pTrx-2. The T7 RNA polymerase of M13mGP1-2 can be used to regulate expression of T7 DNA polymerase by inducing the RNA polymerase with IPTG. M13mGP1-2 (ATCC 40303) is similar to pGP1-2 (ATCC 40175) except that gene 1, T7 RNA polymerase, was ligated (using BglII and SalI linkers) to BamHI-SalI cut M13mp8, placing the gene under control of the lac promoter. |
