| 產品名稱 |
p-rPrl |
| 商品貨號 |
B162687 |
| Designations |
p-rPrl |
| Species |
Rattus norvegicus, rat |
| Depositors |
University of California, Davis, KH Murashige, University of California, Davis |
| Applications |
For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). |
| Vector |
Construct size (kb): 5.190000057220459 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: PRL
Gene name: prolactin
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17
Tissue: anterior pituitary
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.823
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: PRL
Gene name: prolactin
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17
Tissue: anterior pituitary
Type of DNA: cDNA
Insert end: Modification: GC tails
Insert end: Modification: GC tails
Insert size (kb): 0.823
Cross references: Insert lengths(kb): 0.8230000138282776 Tissue: anterior pituitary Gene product: prolactin [PRL] |
| Insert Size (kb) |
0.823 |
| Media |
ATCC® Medium 1083: LB medium for chi 1776
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--5.8; PvuII/PstI--3.0, 1.6, 0.9. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). The insert includes the complete open reading frame plus 5' and 3' untranslated regions. |
| References |
Cooke NE, et al. Human and rat prolactin and preprolactin cloned genes. US Patent 4,725,549 dated Feb 16 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
