| 產品名稱 |
pMAP517 [112-12D] |
| 商品貨號 |
B162712 |
| Designations |
pMAP517 [112-12D] |
| Species |
Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors |
Monsanto Co., FJ Perlak, Monsanto Co. |
| Applications |
produces protein crystal protein (delta endotoxin) |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMAP515
Intact vector size:
Type of vector: plasmid
Vector end: BglII
Vector end: BglII
Cloning sites: BglII
Polylinker sites:
Other unique sites: XhoI
Construction: pSUP1011, pMON7068, pMAP33
Features (with orientation and position when available): |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: Y
Strain: HD-1
Type of DNA: plasmid
Insert 5' end: BamHI
Insert 3' end: BamHI Modification: BamHI -> BglII
Insert size (kb): 4.6
Cross references: Insert lengths(kb): 4.599999904632568 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb) |
4.600 |
| Media |
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The IS50L(kanR/B.t.) gene segment was integrated into the host chromosome by mating E. coli S17-1 containing pMAP517 with P. fluorescens 112-12. The IS50L(kanR/B.t.) gene segment has a +4 frameshift mutation (which converts a wild type XhoI site to a PvuI site) of the transposase gene IS50L. This strain is resistant to 100 mcg/ml rifampicin and 100 mcg/ml nalidixic acid. Strain that proliferates in a plant environment to an extent sufficient to express at least 0.5 ng of insecticidally active protein per each insect to be controlled. |
| References |
Obukowicz MG, et al. Combatting plant insect pests with plant-colonizing microorganisms containing the toxin gene B. thuringiensis as a chromosomal insertion. US Patent 5,229,112 dated Jul 20 1993
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
