| 產(chǎn)品名稱 |
Murid herpesvirus 1 |
| 商品貨號 |
B162738 |
| Classification |
Herpesviridae, Muromegalovirus |
| Deposited As |
Mouse salivary gland virus |
| Agent |
Murid herpesvirus 1 |
| Strain |
Smith MSGV |
| Positive for Mycoplasma |
No |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
Vapor phase of liquid nitrogen |
| Comments |
Virus primarily cell-associated. Cryopreserve virus in 8% DMSO (ATCC® 4-X™). |
| Effect on Host |
CPE, cell rounding and degeneration |
| Recommended Host |
SC-1 cells (ATCC® CRL-1404™) |
| Growth Conditions |
Temperature: 37°C
Recommendations For Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 8 x 104 - 1 x 105 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
|
| Name of Depositor |
WP Rowe, National Institute of Allergy and Infectious Diseases |
| Special Collection |
ATCC |
| Source |
Salivary gland tissues from three naturally infected mice. Derived from VR-194™. Treated to remove Mycoplasma hominis at ATCC®. |
| References |
Selgrade MK, et al. Effects of cell source, mouse strain, and immunosuppressive treatment on production of virulent and attenuated murine cytomegalovirus. Infect. Immun. 33: 840-847, 1981. PubMed: 6270000
Smith MG. Propagation of salivary gland virus of the mouse in tissue cultures. Proc. Soc. Exp. Biol. Med. 86: 435-440, 1954. PubMed: 13194679
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