Replication has not been observed in amphibian or mammalian cell lines.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Whole victim homogenates of trout with infectious pancreatic necrosis, Buhl, Idaho
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Replication has not been observed in amphibian or mammalian cell lines. Brook trout 4 weeks of age die 1-4 weeks after feeding on virus. Stable at -20C (7 yrs) or at 4C (in 50% glycerol).
This preparation has tested positive for A. laidlawii.
Effect on Host
Cytoplasmic contraction and cell lysis
Recommended Host
rainbow trout gonad (RTG-2) cells (ATCC CCL-55)
RTG-2 cells (ATCC CCL-55); other fish cell lines; salmonid fishes (Salvelinus frontionalis, Salmo gairdneri, Sl trutta, S. charki, Salvelinus namaycush, Oncorhynclus kisutch, Salmo salai)
Growth Conditions
Temperature: 21°C
Incubation: 2-3 days
Name of Depositor
KE Wolf, MC Quimby
Source
Whole victim homogenates of trout with infectious pancreatic necrosis, Buhl, Idaho
