Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
contaminated Vero cells, passaged in Israel
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Antigenic cross-reactivity by immunoblot with chlamydial heatshock proteins
Effect on Host
Cytopathic effects (black granulated cytoplasm, "tar spots", developing to numerous vacuoles, with development of "dancing" particles)
Black granulated cytoplasm ("tar spots"), developing to numerous vacuoles, with development of "dancing" particles.
Recommended Host
cells of human and primate origin
Vero (ATCC CCL-81)
Growth Conditions
Incubation: 5 days at 35°C to 37°C in Vero cells (ATCC CCL-81)
Name of Depositor
M Friedman
Special Collection
NCRR Contract
Source
contaminated Vero cells, passaged in Israel
References
Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462
Kahane S, et al. Description and partial characterization of a new Chlamydia-like microorganism. FEMS Microbiol. Lett. 109: 329-333, 1993. PubMed: 8339923
type strain
Cross References
Nucleotide (GenBank) :
AJ012178
Simkania negevensis partial rnpB gene for RNase P RNA subunit.
