Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Rapid loss in titer when stored above -70°C.
Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg/mL Cycloheximide.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
CPE, intracellular inclusion bodies
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, 5% CO2 in air atmosphere is recommended
