| 產(chǎn)品名稱 | Rickettsia monacensis |
|---|---|
| 商品貨號 | B162805 |
| Classification | Rickettsiaceae, Rickettsia |
| Deposited As | Rickettsia monacensis |
| Agent | Rickettsia monacensis |
| Strain | IrR/Munich |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | Vapor phase of liquid nitrogen |
| Genome Sequenced Strain | Rickettsia monacensis, strain IrR/Munic, complete genome. |
| Images |  |
| Comments | Genome sequenced by depositor |
| Effect on Host | CPE, refractile rounding, sloughing, and degeneration |
| Recommended Host | Vero cells (ATCC® CCL-81™) |
| Growth Conditions | Temperature: 34°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 65-75% confluent. Remove DMSO from bacteria vial by centrifugation (180 x g for 10 minutes at 4°C). Remove supernatant and add 2 mL of fresh medium to the pellet. Pulse vortex for 1 minute. Remove medium from host cell culture and inoculate with bacteria (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1 to 1). Adsorb by centrifugation for 30 minutes at 800 x g at 22°C. Add agent growth medium following adsorption. Incubation: 6-11 days at 34°C in a humidified atmosphere, until CPE is progressed through 80% of the monolayer. A Giemsa stain may also be used to determine end of incubation. |
| Name of Depositor | T Kurtti, University of Minnesota |
| Chain of Custody | ATCC <-- T Kurtti |
| Source | Tick (Ixodes ricinus), Munich, Germany. Isolated on June 12, 1998. |
| Year of Origin | 1998 |
| References | Simser JA, et al. Rickettsia monacensis sp. nov., a spotted fever group Rickettsia, from ticks (Ixodes Ricinus) collected in a European city park. Appl Environ Microbiol 68(9): 4559-4566, 2002. PubMed: 12200314 |
| Cross References | Nucleotide (GenBank) : NZ_LN794217.1 Rickettsia monacensis, strain IrR/Munic, complete genome. |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
