| 產品名稱 | Human rhinovirus 5 |
|---|---|
| 商品貨號 | B162806 |
| Classification | Picornaviridae, Enterovirus, Rhinovirus B |
| Agent | Human rhinovirus 5 |
| Strain | Norman |
| Applications | Respiratory research |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | ATCC® 1927™ was derived from ATCC® 485™ following treatment with diethyl ether to remove Mycoplasma to remove mycoplasma contamination. REFERENCE STRAIN: Collaborative Report on Rhinoviruses, Nature No. 5078, 213:761, 1967. Also for types 13-17: Panel for Picornaviruses, Melnick, J. et al., Science 141:153, 1963. |
| Effect on Host | CPE, rounding and sloughing |
| Recommended Host | WI-38 cells (ATCC® CCL-75™) |
| Growth Conditions | Temperature: 33.5°C Recommendations for Infection: Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously. End adsorption by adding virus growth medium. Incubation: 2-4 days at 33.5°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | VV Hamparian, RM Conant |
| Source | Human throat washings |
| References | Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011 Palmerberg AC and Gern JE. Classification and evolution of human rhinoviruses. Methods Mol Biol. 1221:1-10, 2015. doi: 10.1007/978-1-4939-1571-2_1. PubMed: 25261302 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
