| 產品名稱 | Mumps rubulavirus |
|---|---|
| 商品貨號 | B162814 |
| Classification | Paramyxoviridae, Rubulavirus |
| Agent | Mumps rubulavirus |
| Strain | MuV/Iowa.US/2006 (Genotype G) |
| Common Name | Mumps virus |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Genome Sequenced Strain | Mumps virus strain MuV-IA, complete genome |
| Images |  |
| Effect on Host | CPE, cell fusion and sloughing |
| Recommended Host | Vero E6 cells (ATCC® CRL-1586™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001-0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium. Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | P Rota, CDC |
| Source | Isolated by oral swab from a human in Iowa, USA, 2006 |
| References | Boddicker JD, et al. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens. J Clin Microbiol. 45(9): 2902-2908, 2007. PubMed: 17652480 Xu P, et al. Rescue of wild-type mumps virus from a strain associated with recent outbreaks helps to define the role of the SH ORF in the pathogenesis of mumps virus. Virology 417(1): 126-136, 2011. PubMed: 21676427 |
| Cross References | Nucleotide (GenBank) : JN012242.1 Mumps virus strain MuV-IA, complete genome Nucleotide (GenBank) : DQ661745.1 Mumps virus strain MUM/Iowa.US/2006 small hydrophobic protein mRNA, complete cds |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
