| 產品名稱 | Human rhinovirus 50 |
|---|---|
| 商品貨號 | B162830 |
| Classification | Picornaviridae, Enterovirus, Human rhinovirus A |
| Deposited As | Rhinovirus 50 |
| Agent | Human rhinovirus 50 |
| Strain | A2#58 |
| Applications | Respiratory research |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | ATCC® VR-1876™ was derived from ATCC® VR-517™ following treatment with diethyl ether to remove mycoplasma contamination. |
| Effect on Host | CPE, refractile rounding and sloughing |
| Recommended Host | HeLa cells (ATCC® CCL-2™) |
| Growth Conditions | Temperature: 33°C Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere while rocking. End adsorption by adding virus growth medium. Incubation: 6-7 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | VV Hamparian, RM Conant |
| Chain of Custody | ATCC <-- VV Hamparian, RM Conant |
| Source | Human throat washings |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
