Human and other primate B-lymphocyte transformation In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
ATCC® VR-1492PQ™ is a high-titer, purified, live virus suspended in 10 mM Tris-HCL (pH 7.5) with 135 mM NaCl, 0.5% BSA, and 5% Trehalose. This product was prepared from ATCC® VR-1492™ via purification by tangential filtration.
This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™).
Quality control testing is done by transformation assay instead of TCID50.
Effect on Host
EBV transforms/immortalizes human and other primate B-lymphocytes. Indicators of transformed cells include cone-shaped cells, cells with spike-like projections, and small clusters of healthy cells.
Recommended Host
B95-8 cells
Growth Conditions
Temperature: 37°C
Recommendations for Infection: This EBV preparation is capable of transforming B-lymphocytes from a preparation of normal human lymphocytes when co-cultivated with a feed layer culture (such as ATCC® 55-X™).
Incubation: 10-25 days at 37°C in a humidified 5% CO2 atmosphere, until indicators of successful transformation appear.
Name of Depositor
G Miller
Source
Human lymphoblastoid cell line (883L) derived from a patient with transfusion-induced infectious mononucleosis.
References
Caputo JL, et al. An effective method for establishing Human B lymphoblastic cell lines using Epstein-Barr Virus. J. Tissue Culture Methods 13: 39-44, 1991.
Miller G, et al. Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proc. Natl. Acad. Sci. USA 69: 383-387, 1972. PubMed: 4333982
Tumilowicz JJ, et al. Presence of retrovirus in the B95-8 Epstein-Barr virus-producing cell line from different sources. In Vitro 20: 486-492, 1984. PubMed: 6086497
