Cells which have been infected with this construct can be transfected by plasmids which contain a gene targeted for expression inserted between a T7 promoter and T7 terminator. Expresses bacteriophage T[7] RNA polymerase. Recombinant virus constructed in vitro. Expresses bacteriophage T[7] RNA polymerase. Expression of the T7 RNA polymerase is directed by the P7.5 promoter which includes early and late regulatory signals. Constructed by transfecting vaccinia virus-infected cells with pTF703 (ATCC 67202). |
