Transfer infected cells once a week at 1:6 ratio; peak virus production occurs 48-72 hours after transfer.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
This persistently infected cell line was established from an excised mammary tumor (adenocarcinoma) of a GR mouse, 1972
Product Format
frozen
Storage Conditions
Liquid nitrogen
Comments
The GR mammary tumors appear in infected GR mice during pregnancy and disappear at the end of pregnancy.
Transfer infected cells once a week at 1:6 ratio; peak virus production occurs 48-72 hours after transfer.
Effect on Host
No, in vitro effects: cytopathic effects in tissue culture
Yes, tumorigenicity: produces hyperplastic alveolar nodules, plaques and mammary tumors in mice under suitable hormonal conditions
Recommended Host
mouse
mouse; mouse in tissue culture
Growth Conditions
Incubation: 2-3 days
Name of Depositor
EY Lasfargues
Special Collection
ATCC
Source
This persistently infected cell line was established from an excised mammary tumor (adenocarcinoma) of a GR mouse, 1972
Year of Origin
1972
References
Muhlbock O. Note on a new inbred mouse-strain GR-A. Eur. J. Cancer 1: 123-124, 1965. PubMed: 5874925
Ringold G, et al. Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization. Virology 65: 135-147, 1975. PubMed: 49975
Lasfargues E. . J. Cell Biol. 59: 187a and 373, 1973.
