| 產品名稱 |
Adeno-associated virus 2 |
| 商品貨號 |
B163073 |
| Classification |
Parvoviridae, Dependovirus, Adeno-associated virus-2 |
| Deposited As |
Adeno-associated virus type 2 |
| Agent |
Adeno-associated virus 2 |
| Strain |
H |
| Applications |
Resistant to heat 56°C 4 hours; this property can be used to selectively inactivate the helper adenovirus - Human adenovirus 2 (ATCC® VR-846™)
Cross-reacts with AAV-3; no hemagglutinin detected |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Depends upon helper virus Human adenovirus 2 ATCC® VR-846™ for replication. Helper virus may be inactivated at 56°C for 30 minutes.
Resistant to heat 56°C 4 hours; this property can be used to selectively inactivate the helper adenovirus. Resistant to ether and chloroform.
Cross-reacts with AAV-3; no hemagglutinin detected. Depends upon helper adenovirus for replication.
|
| Effect on Host |
Adenovirus CPE, refractile rounding and cell sloughing.? AAV shows no CPE. |
| Recommended Host |
HEK-293 cells (ATCC® CRL-1573™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus and helper virus(e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1.0 each for virus and helper virus). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 2-3 days at 37°C in a humidified 5% CO2 atmosphere, until helper virus CPE is progressed through 80-90% of the monolayer. AAV does not cause CPE. |
| Name of Depositor |
MD Hoggan |
| Source |
Adenovirus type 12 strain 97838 in KB cells |
| References |
Hoggan MD, et al. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc. Natl. Acad. Sci. USA 55: 1467-1474, 1966. PubMed: 5227666
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