Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
derived from existing strain
Product Format
frozen
Storage Conditions
-70°C or colder
(or colder)
Comments
This an encephalitogenic strain of Canine parainfluenza virus.
Effect on Host
Yes, in vitro effects: cytopathic effect, syncytial sloughing
CPE, syncytial sloughing
Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host
Recommended Host:? ATCC® CCL-81™ (Vero)
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 36.0°C
Duration: 3-4 days; For best results cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent).
Cryopreservation
Storage temperature: -70°C
Name of Depositor
ATCC, JF Evermann
Special Collection
ATCC
Source
derived from existing strain
References
Evermann JF, et al. Isolation of a paramyxovirus from the cerebrospinal fluid of a dog with posterior paresis. J. Am. Vet. Med. Assoc. 177: 1132-1134, 1980. PubMed: 6254938
Evermann JF, et al. Properties of an encephalitogenic canine parainfluenza virus. Arch. Virol. 68: 165-172, 1981. PubMed: 6168243
or colder
ATCC VR-963 was filtered to remove bacterial and fungal contaminants and reaccessioned as ATCC VR-1573. ATCC VR-963 was isolated from cerebrospinal fluid from dog with posterior paresis.
