| 產品名稱 |
Gallid herpesvirus 3 |
| 商品貨號 |
B163137 |
| Classification |
Herpesviridae, Mardivirus |
| Deposited As |
Marek's disease virus type 2 |
| Agent |
Gallid herpesvirus 3 |
| Strain |
SB-1 (clone of SB) |
| Applications |
Will protect against MDV and non-virus producing JM tumor transplant. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
chicken (S-strain) peripheral blood lymphocytes |
| Product Format |
frozen |
| Storage Conditions |
?Vapor phase of liquid nitrogen |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
Non-oncogenic, cell-associated. Will protect against MDV and non-virus producing JM tumor transplant. Note that SB-1 is strictly cell-associated, damage to the infected cells will cause loss of titer. Thaw frozen ampule in 27C waterbath; dilute content slowly in 9.0 ml medium or PBS; pellet cells (10min at 400Xg); resuspend in culture medium. |
| Effect on Host |
?CPE, cell rounding and syncytia formation |
| Recommended Host |
chicken embryo fibroblasts |
| Growth Conditions |
Temperature: 38°C to 39°C Incubation: ?Incubate infected culture for 7 days at 38-39°C in a humidified 5% CO2 atmosphere, until CPE are well advance throughout. |
| Cryopreservation |
Storage temperature: Liquid nitrogen |
| Name of Depositor |
BW Calnek |
| U.S. Patent |
|
| U.S. Patent Number |
|
| Source |
chicken (S-strain) peripheral blood lymphocytes |
| References |
Schat KA, Calnek BW. Characterization of an apparently nononcogenic Marek's disease. J. Natl. Cancer Inst. 60: 1075-1082, 1978. PubMed: 76680
|
