| 產品名稱 |
Vaccinia virus |
| 商品貨號 |
B163285 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus |
| Strain |
rVV-hgp100 |
| Applications |
VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
VR-1605 is recombinant Vaccinia virus strain rVV-hgp100 that expresses human melanoma antigen gp100. It is considered a potent immunogen to activate tumor reactive T cells in both human and mice, and was developed by NCI. |
| Effect on Host |
Cell enlargement and clustering of rounded cells in cell culture; plaques with rounding and eventual sloughing. |
| Recommended Host |
Production Host: Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts)). Production Host: ?Human TK- cells (provided by the depositor (Dr. Zhiya Yu). Virus can be propagated in numerous cell lines (see Alternate Hosts).) Alternate Hosts: BS-C-1 cells (ATCC CCL-26), Vero cells (ATCC CCL-81), and HeLa cells (ATCC CCL-2) |
| Growth Conditions |
Plant cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Process virus by freeze/ thawing it three times by vapor phase of liquid nitrogen and 37.0°C water bath (vortex mixing on high speed for 20 seconds between each freeze/ thaw), sonicating with 40% power for 30 seconds, and then a final vortex mixing on high speed for 30 seconds. Remove cell growth medium from the flask, and inoculate with a small volume of virus stock diluted in virus growth medium to provide a MOI of about 1-3. Adsorb 1-2 hours at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubate infected culture for 1-3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture. |
| Name of Depositor |
Dr. Zhiya Yu from Surgery Branch/ NCI/ NIH |
| Source |
rVV-hgp100 was constructed in Dr. Nicolas P. Restifo's laboratory of Surgery Branch/ NCI/ NIH in the early 1990's. |
