| 產品名稱 |
Vaccinia virus ts mutant |
| 商品貨號 |
B163317 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts30 |
| Applications |
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D4, encoding a viral uracyl DNA glycosylase. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This conditional lethal mutant, originally designated plaque isolate no. 4149, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B indicating that it induces foci of a fibrous viroplasmic matrix substance in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D4, encoding a viral uracyl DNA glycosylase. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
This conditional lethal mutant, originally designated plaque isolate no. 4149, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B indicating that it induces foci of a fibrous viroplasmic matrix substance in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D4, encoding a viral uracyl DNA glycosylase. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses |
| Effect on Host |
CPE: enlargement, plaques with rounding and eventual sloughing Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing. |
| Growth Medium |
Fetal Bovine Serum
ATCC® Medium 30-2002: Dulbecco's Modified Eagle's Medium (DMEM)
|
| Recommended Host |
Recommended Host: BSC-40 (ATCC CRL-2761) Production host: BSC-40 (ATCC CRL-2761)
Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2 |
| Name of Depositor |
S. Dales, R. Condit |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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