Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
This item is a mycoplasma free preparation derived from VR-583 following treatment to remove mycoplasma. VR-583 will no longer be distributed when current inventory is depleted. Strain DN-19 is considered to be an antigenic variant or "prime" of Human coxsackievirus A 24. It was originally known as Echovirus 34.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
This item is a mycoplasma free preparation derived from VR-583 following treatment to remove mycoplasma. VR-583 will no longer be distributed when current inventory is depleted. Strain DN-19 is considered to be an antigenic variant or "prime" of Human coxsackievirus A 24. It was originally known as Echovirus 34.
Effect on Host
CPE in culture include highly refractile, rounded cells throughout monolayer or in loose clusters
CPE in culture include highly refractile, rounded cells throughout monolayer or in loose clusters.
Growth Medium
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: WI-38 (ATCC CCL-75)
Recommended Host: ?WI-38 cells (ATCC?CCL-75™)
Alternate Hosts: Human embryonic kidney cells, human aorta cells (A-39)
Growth Conditions
For best results, plate cells 24-48 hours in advance. Infect at 80%-90% confluence using a multiplicity of infection (MOI) of 0.1 to 1.0. Incubate at 37.0°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. Harvest cells and culture fluids; cell debris can be removed by centrifugation if desired.
Name of Depositor
HA Wenner
Source
Isolated from infant with diarrhea, Texas, 1963; derived from ATCC VR-583 following treatment to remove mycoplasma contamination.
References
The Work of WHO Virus Reference Centers and the Services they Provide, Geneva. Bull. World Health Organ 11: 1968.
Schmidt NJ, Lennette EH. Antigenic variants of coxsackievirus type A24. Am. J. Epidemiol. 91: 99-109, 1970. PubMed: 4313417
Rosen L et al. Subclassification of enteroviruses and ECHO virus type 34. Brief report. Archives of Virology 30: 89-92, 1970. PubMed: 4986680.
Brown BA. Resolving ambiguities in genetic typing of human enterovirus species C clinical isolates and identification of enterovirus 96, 99 and 102. J. Gen. Virol. 90 (PT 7): 1713-1723, 2009. PubMed: 19264596
