Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1.0 mL per vial
Storage Conditions
-70°C or colder
Comments
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 rpm (750 x g) for one hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 µg /mL cycloheximide. Incubate at 36°C for 3 days.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent).
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Name of Depositor
SP Wang
Source
Human cervix (epithelial tissue), cervicitis, Seattle, WA, 1971
References
Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875
Fong CK, et al. Re-examination of the McCoy cell line for confirmation of its mouse origin: karyotyping, electron microscopy and reverse transcriptase assay for endogenous retrovirus. Clin Diagn Virol 2(2): 5-103, 1994. PubMed: 15566757
