| 產品名稱 |
Simian adenovirus 7 |
| 商品貨號 |
B163368 |
| Classification |
Adenoviridae, Mastadenovirus |
| Deposited As |
SV25 |
| Agent |
Simian adenovirus 7 |
| Strain |
8045-2WN |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Simian Virus Group I (Hull).
REFERENCE STRAIN: Pereira, H.G. et al., Virology 20:613, 1963.
Originally deposited as SV25 |
| Effect on Host |
CPE, refractile rounding and sloughing |
| Recommended Host |
LLC-MK2 (ATCC CCL-7.1) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-14 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor |
RN Hull |
| Source |
Tissue cultures prepared from pooled rhesus monkey kidney cells |
| References |
Minner JR, et al. New viral agents recovered from tissue cultures of monkey kidney cells. III. Recovery of additional agents both from cultures of monkey tissues and directly from tissues and excreta. Am. J. Hyg. 68: 31-44, 1958. PubMed: 13559209
Roy S, et al. Construction of gene transfer vectors based on simian adenovirus 7. J. Gen. Virol. 92(8): 1749-1753, 2011. PubMed: 21562118
|
| Cross References |
Nucleotide (GenBank) :
DQ792570.1
Simian adenovirus 7, complete genome
|
