Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae.
Biosafety Level
3
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
blood of patient with oriental spotted fever, Japan
Product Format
frozen
Storage Conditions
Liquid nitrogen freezer
Comments
Species-specific monoclonal antibody only reacts with R. japonica but not with other spotted fever group rickettsiae. Vero cells demonstrated to be mycoplasma-free before infection.
Infected Vero cells grow like uninfected cells in MEM with 10% FBS devoid of antibiotics. Subculture by dispersion with 0.05% trypsin and 0.02% EDTA is possible for many passages.
Restrictions: This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit.
blood of patient with oriental spotted fever, Japan
References
Uchida T, et al. Rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in Japan. Int. J. Syst. Bacteriol. 42: 303-305, 1992. PubMed: 1581190
Uchida T, et al. Isolation of a spotted fever group Rickettsia from a patient with febrile exanthematous illness in Shikoku, Japan. Microbiol. Immunol. 30: 1323-1326, 1986. PubMed: 3553872
