| 產品名稱 |
Vaccinia virus ts mutant |
| 商品貨號 |
B163388 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts50 (Dales isolate 7014) |
| Applications |
The non-permissive incubation temperature is 39.5oC. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U10. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. |
| Positive for Mycoplasma |
No |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Not described in the original publication. Presumed to have been selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U10. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). |
| Effect on Host |
Yes, Cytopathic effect: Rounding and sloughing of infected cells CPE: enlargement, plaques with rounding and eventual sloughing |
| Recommended Host |
Recommended Host: BSC-40 (ATCC CRL-2761 |
| Growth Conditions |
Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent]. |
| Name of Depositor |
S Dales, RC Condit, S Dales |
| Special Collection |
NCRR Contract |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
Non-permissive incubation temperature is 39.5C. This mutant was not assigned a Dales EM category. It is assigned the Condit map location U10. This subtype is distinguishable from the parent IHD-J strain by its ability to elicit polykaryocytosis in all mammalian and avian cells tested and by its inability to induce an active hemagglutinin at the plasma membrane.
cell enlargement, plaques with rounding and eventual sloughing of cells
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
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