Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Addition of 1 μg/mL trypsin (1:250 Gibco trypsin catalog #27250018) to culture medium is required for optimal growth. Agglutinates both chicken and guinea pig RBC (0.5%) but the latter to a higher titer than the former. Based on electron microscopic evidence, this product may also contain an as yet unidentified polyoma virus.
Virus presence can be detected with Millipore #5019 Parainfluenza 1 Antibody FITC Reagent
Effect on Host
Slight CPE, rounding and sloughing
Recommended Host
LLC-MK2 cells (ATCC® CCL-7.1™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-7 days continuously rocking at 37°C in a humidified 5% CO2 atmosphere.
Name of Depositor
R Huebner, RM Chanock, JC Hierholzer, A Chappell
Source
Throat swab, 3-year-old boy with acute laryngitis, Washington, DC, 1957
References
Andrewes CH, et al. Para-influenza viruses 1, 2, and 3: Suggested names for recently described myxoviruses. Virology 8: 129-130, 1959. PubMed: 13669331
Chanock RM. . N. Engl. J. Med. 258: 207-213, 1958.
