Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Centrifuge at 2000 X g for 1 hour; adsorb 1 hour at 35-36°C. Feed with 1.0 mL EMEM (HEPES buffered) 5mg glucose/mL, 0.3 mg glutamine/mL, 1-2 µg/mL cycloheximide. Dilute high titer stocks to avoid cell toxicity.
Tissue culture adapted at ATCC.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies.
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: For best results cells should be 24 to 48 hours old and 80-90% confluent.
Incubation: 2-3 days, a 5% CO2 in air atmosphere is recommended
Name of Depositor
J Schachter
Source
Lymph node from human with LGV
References
Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618
Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529
Cross References
Nucleotide (GenBank) :
AJ012175
Chlamydia trachomatis partial rnpB gene for RNase P RNA subunit.
