| 產品名稱 |
10B9 |
| 商品貨號 |
B163536 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with human umbilical vein endothelial cells and the immature macrophage cell line, U937. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human endothelium |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human endothelium |
| Comments |
Animals were immunized with human umbilical vein endothelial cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with human umbilical vein endothelial cells and the immature macrophage cell line, U937. It does not react with bovine pulmonary or aortic endothelial cells, human monocytes or mature cultured macrophages or DHL-2 cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 85%; fetal bovine serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
YA Reid, AW Hamburger |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hamburger AW, et al. Isolation and characterization of monoclonal antibodies reactive with endothelial cells. Tissue Cell 17: 451-459, 1985. PubMed: 3901396
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