| Comments |
The 10P12 cell line was established by transformation of placental cells from a 10 day mouse embryo with A-MuLV. The cells are surface immunoglobulin positive, B23.1 positive, B220 positive and possess receptors for IgE. This line represents a transformed stage of a very early hematopoietic precursor and shares surface antigens with multipotent stem cells. Transcripts of germline immunoglobulin and T cell receptor genes are detectable. |
| References |
Siegel ML, et al. Isolation and characterization of Abelson murine leukemia virus- transformed mast cell lines from midgestation embryonic placenta. Eur. J. Immunol. 15: 1136-1141, 1985. PubMed: 2998805
Siden EJ. Regulated expression of germline antigen receptor genes in mast cell lines from the murine embryo. J. Immunol. 150: 4427-4437, 1993. PubMed: 7683318
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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