| 產品名稱 |
1116-NS-19-9 |
| 商品貨號 |
B163539 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma,Cancer |
| Applications |
The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Clinical Data |
The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against colorectal carcinoma monosialoganglioside |
| Cellular Products |
immunoglobulin; monoclonal antibody; against colorectal carcinoma monosialoganglioside |
| Comments |
Animals were immunized with the SW1116 human colon carcinoma cell line (ATCC CCL-233). Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody binds to human tumors of endodermal origin (gastric, pancreatic and colorectal carcinomas) and detects the antigen in serum and urine of cancer patients. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
Wistar Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Steplewski Z, et al. Detection of colorectal carcinoma. US Patent 4,471,057 dated Sep 11 1984
Koprowski H, et al. Colorectal carcinoma antigens detected by hybridoma antibodies. Somatic Cell Genet. 5: 957-971, 1979. PubMed: 94699
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