| 產品名稱 |
116-13.1 |
| 商品貨號 |
B163544 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Lyt-2.1 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Lyt-2.1 |
| Comments |
Animals were immunized with spleen cells and thymocytes from CE mice. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a |
| Name of Depositor |
F Shen |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
. Monoclonal antibodies and T-cell hybridomas. Amsterdam: Elsevier; 1981.
|
