| 產品名稱 |
12/44 |
| 商品貨號 |
B163550 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody is cytotoxic for BALB/c male lymph node cells and cross-reacts with human H-Y. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Clinical Data |
Female BALB/c mice were immunized with lymphocytes from male BALB/c mice. The antibody is cytotoxic for BALB/c male lymph node cells and cross-reacts with human H-Y. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against H-Y antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against H-Y antigen |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Female BALB/c mice were immunized with lymphocytes from male BALB/c mice. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody is cytotoxic for BALB/c male lymph node cells and cross-reacts with human H-Y. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM; kappa light chain |
| Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Koo GC, et al. Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen. US Patent 4,680,258 dated Jul 14 1987
|
