| 產品名稱 |
132-1C8 |
| 商品貨號 |
B163562 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Gender |
male |
| Strain |
129GIX+ |
| Applications |
The antibody reacts with the synthetic MYB oncogene peptide. In BM2 cells, proteins of 50 kd and 20 kd are detected. In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the synthetic MYB oncogene peptide. In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected. |
| Clinical Data |
male |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a synthetic MYB oncogene peptide |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a synthetic MYB oncogene peptide |
| Comments |
Animals were immunized with a synthetic polypeptide with the sequence RHYDEDPEKEKRIKELEL corresponding to peptides 282 to 300 of the MYB oncogene. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with the synthetic MYB oncogene peptide. In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
IgG1 kappa |
| Name of Depositor |
National Cancer Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Animals were immunized with the synthetic peptide RHYDEDPEKEKRIKELEL corresponding to residues 282 through 300 of the MYB sequence (formerly residues 94 through 112 of the v-myb sequence).
In immunoblot assays, the antibody recognizes proteins of approximately 80 kd and 60 kd in extracts from MSTF cells (mink lung cells transformed by fes). In human tumor (MOLT 4), a 60 kd protein is detected as well as two proteins of 40 and 45 kd. These two proteins of 40 and 45 kd are also detected in human brain extracts. In BM2 cells, proteins of 50 kd and 20 kd are detected.
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