| 產品名稱 |
132C4A/4 |
| 商品貨號 |
B163563 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni |
| Cellular Products |
immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni |
| Comments |
Animals were immunized with cercariae glycoprotein from Schistosoma mansoni. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Complete Growth Medium |
Culture Medium: Modified Dulbecco's medium (see below) with HAT, 90%; fetal
bovine serum, 10%.
DME w/HEPES (10mM) 100 mL
L-Glutamine (100x) 1 mL
Solution I 1 mL
Non-essential amino acids (100x) 1 mL
NCTC 135 10 mL
Fetal Bovine Serum 12 mL
Solution I (100x)
1. 1320 mg oxalacetic acid (100 mM, MW 132).
2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg).
Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to
100 mL with distilled water. Stir at 37°C until solution dissolves. Filter,
aliquot and store frozen. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium, 40%; fetal bovine serum, 50%; DMSO, 10% |
| Isotype |
IgG1 |
| Name of Depositor |
Johns Hopkins University |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. US Patent 4,416,866 dated Nov 22 1983
|
