| Comments |
Animals were immunized with purified shiga-like toxin from E. coli H30. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the beta subunit of shiga toxin and SLTI and neutralizes Shiga toxin activities from Shigella dysenteriae type I and Shigella flexneri and Shiga-like toxin activities from Escherichia coli. Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhimurium. Tested and found negative for ectromelia virus (mousepox). |
| References |
Strockbine NA, et al. Characterization of monoclonal antibodies against shiga-like toxin from Escherichia coli. Infect. Immun. 50: 695-700, 1985. PubMed: 3905611
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
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Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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