| 產品名稱 |
14.8 |
| 商品貨號 |
B163567 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Applications |
This antibody reacts with the CD45R antigen which is preferentially expressed on B cells and their precursors and a subpopulation of T cells. The antibody binds to the high molecular weight isoform of CD45 expressing all three variable exons. The antibody is important because it detects B cell precursors in 16 day old fetal mouse liver, and plates coated with this antibody have been used for depletion experiments. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
Tested and found negative for ectromelia virus (mousepox). immunoglobulin; monoclonal antibody; against mouse B cell (B lymphocyte) precursors (CD45RA),The epitope recognized requires expression of exon A. |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse B cell (B lymphocyte) precursors (CD45RA) |
| Comments |
Animals were immunized with W279B murine B lymphoma cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. This antibody reacts with the CD45R antigen which is preferentially expressed on B cells and their precursors and a subpopulation of T cells. The epitope recognized requires expression of exon A. The antibody binds to the high molecular weight isoform of CD45 expressing all three variable exons. Thy-1 positive peripheral T cells are positive with this antibody. The antibody is important because it detects B cell precursors in 16 day old fetal mouse liver, and plates coated with this antibody have been used for depletion experiments. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG2b |
| Name of Depositor |
PW Kincade |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Kincade PW, et al. Antigens displayed on murine B lymphocyte precursors. J. Immunol. 127: 2262-2268, 1981. PubMed: 6975314
Landreth KS, et al. Enrichment of human marrow lymphocytes with monoclonal antibodies to murine antigens. Proc. Natl. Acad. Sci. USA 79: 2370-2374, 1982. PubMed: 7048318
Luqman M, et al. Differential expression of the alternatively spliced exons of murine CD45 in Th1 and Th2 cell clones. Eur. J. Immunol. 21: 17-22, 1991. PubMed: 1671357
Kincade PW. Formation of B lymphocytes in fetal and adult life. Adv. Immunol. 31: 177-245, 1981. PubMed: 7032254
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