| 產品名稱 |
143B PML BK TK |
| 商品貨號 |
B163574 |
| Organism |
Homo sapiens, human |
| Tissue |
bone |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
osteosarcoma |
| Age |
13 year old |
| Gender |
female |
| Ethnicity |
Caucasian |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Clinical Data |
female Caucasian 13 year old |
| Genes Expressed |
genome, partial [tk] |
| Comments |
Contains a thymidine kinase (TK+) plasmid (PMBKTK). |
| Complete Growth Medium |
Minimum essential medium (Eagle) in Earle's BSS with HAT (0.1 mM hypoxanthine, 400 nM aminopterin, 0.16 mM thymidine), 90%; fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 11 vWA: 18 |
| Name of Depositor |
Wistar Institute |
| U.S. Patent Number |
|
